Fast protein liquid chromatography (FPLC) Term Paper

Fast protein liquid chromatography (FPLC) - Term Paper Example Furthermore, it supposes that the chromatographic column contains an infinite number of separate layers (theoretical plates). Separate equilibrations of the sample between the stationary and mobile phase occur in these layers. The analyte moves down the column by transfer of equilibrated mobile phase from one ‘plate’ to the next. There is a more convincing theory, ‘the rate theory.’ This theory depends on the speed of elution and thus speeds of diffusion of the dissolved particles. The analysis and application of this theory leads to the Van Deemter equation. This equation relates the variance per unit length of a separation column to the linear mobile phase velocity by considering several factors. They are physical, kinetic, and thermodynamic properties of a separation. The physical factors are such as; A) Eddy diffusion. B) Longitudinal diffusion C) Resistance to mass transfer It (chromatography) is thus seen to exploit the differences in partitioning beha vior between a mobile phase and a stationary phase to separate the components in a mixture. These components contained within a mixture may interact with the stationary phase based on charge, differing solubility or adsorption capability. Several terminologies are associated with the process of chromatography; a) The analyte- this is the substance to be separated during chromatography. b) Bonded phase- this is a stationary phase that is covalently bonded to the support particles or to the inside wall of the tube being utilized.. c) A chromatogram is the visual output of the chromatograph. d) The eluate is the mobile phase that is leaving the separation column. e) The eluent is the solvent that carries/dissolves the analyte. f) The immobilized phase is a stationary phase that is immobilized on the support particles, or on the inner wall of the column tubing. It is similar to the bonded phase g) The mobile phase is the phase that moves in a definite direction. h) The  solute  refe rs to the sample components in a solvent. i) The  solvent  refers to any substance capable of solubilizing another substance. This is important especially in the liquid mobile phase in liquid chromatography. Several methods of chromatography exist as well (singh). They include; 1) Chiral chromatography 2) Countercurrent chromatography 3) Pyrolysis gas chromatography 4) Simulated moving bed chromatography 5) Reversed phase chromatography 6) Two dimensional chromatography 7) Expanded bed adsorption chromatography 8) Size exclusion chromatography 9) Ion exchange chromatography 10) Supercritical fluid chromatography 11) FPLC The FPLC is the method of interest in this case. The FPLC method was developed and marketed in Sweden by the Pharmacia Company in 1982. It was originally called fast performance liquid chromatography. Principle of functioning The purpose of purifying proteins with FPLC is to deliver quantities of the target protein at sufficient purity. This is done in a way tha t ensures the protein is in a biologically active state to suit its further use. Furthermore this can mean pure enough that the biological activity of the target is retained. This high level of purity requires preliminary preparation of the sample. This is mostly by IEC. In most FPLC systems, there are two solvents/ buffers (A, B). There is also a resin that is chosen so that the protein of interest will bind to it by a charge interaction. When the sample and mix of buffer (100% A) and protein is introduced, the protein will bind to

Environmental Challenges that Sony Faces Essay Example | Topics and Well Written Essays - 3000 words

Environmental Challenges that Sony Faces - Essay Example The present research has identified that various changes in the marketplace which particularly should always involve political, economic, social, technological, environmental and legal factors and more. Under political factor, it is always common to look at the government’s conservatism when it comes to dealing with business. One perfect example would be the case of privatization. In every government, there is always a strong stand on controlling the rise of the certain organization. In Japan, for instance, various organizations are faced with remarkable challenges because the government is trying to protect the interest of small organizations in their respective industry. However, the ability of Sony to leverage its core competencies together with other organization is a proof that there is always the right timing in politics. For instance, Sony’s intent to leverage its core competencies with a Swedish telecommunication company in order to beat Nokia resulted in the bi rth of Sony Ericsson. This joint venture is a remarkable proof that finding the right place for each organization is needed in order to enhance its core competencies. This at some point has specifically involved the prevailing political stand of a nation which basically has a great impact on the entire business process of an organization. For instance, a government saying no to privatization may significantly create an impact on those organizations planning to leverage their core competencies through it. With this, it is important to understand politics as a major thing that affects how the entire business should be conducted primarily because of the prevailing government’s stand. However, it is not only in this broad and general illustration how politics essentially affects Sony. In modern situation, politics itself could stand between competitors or even within an organization. This is a proven trend in the whole business industry of electronics. For instance, every profit organization in this industry is simply trying to be a cut above the other by reaching its competitive advantage. Competitive advantage is something that needs to be worked out because it serves as something that will enhance core competencies.